I am running the counting of tryp today. So i have my practical student - Wai Yan and some friend to help. Really want to thank them fro helping.
wa.... really want to vomit for seeing under 40X microscope! I need to count using hemocytoeter, mean i need to count : 16 (kotak kecil)X 4 (replications) X 6 (samples) = 384 kotak kecil. Each 16 otak kecil contain ~150 tryp, so 384 X 150 = 57600 ekor tryp!!! I surrender after counted 4 samples! my eyes were so tired! but still have to carried on... take a 10 minutes la...
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